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Differential-Display Reverse Transcription-PCR (DDRT-PCR) (Springer Lab Manuals)
by Sergio Colonna-Romano ; Antonella Leone ; Bruno Maresca
Binding: Paperback, 1 edition, 124 pages
Publisher: Springer
List Price: USD $89.95
Weight: 61
Dimension: H: 0.75 x L: 9.5 x W: 0.53 inches
ISBN 10: 3540632972
ISBN 13: 9783540632979
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Book Description:
Identification of differentially expressed genes is one of the major challenges in molecular biology. Several techniques allow the cloning of such sequences. However, methods such as RNA subtraction or differential hybridization are time consuming and require large amounts of mRNA. Recently, a new approach has successfully been developed: Differential Display Reverse Transcription PCR (DDRT PCR). This technique has been proven to be highly effective in identifying sequences that are differentially expressed in various cell types. The most striking advantage is, however, that only nanograms of total RNA are sufficient. Thus every mRNA species expressed in the cell system can be investigated, even those present at very low levels.

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