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Book Description: Understanding GENE FUNCTION and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. Gene Transfer; Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring appropriate expression. The detailed, step by step protocols cover a variety of methods, both well established and newly evolving. These includeviral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. Each chaper includes technical discussions for selectionof the appropriate system and strategies for delivery. Related Titles from the Publisher Binding and Kinetics for Molecular Biologists; DNA Replication and Human Disease; DNA Science; Epigenetics; Genes and Signals; Genetic Variation; Genomes; Molecular Biology of the Gene, 6th Edition; Recombinant DNA: Genes and Genomes - A Short Course

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