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The PCR Technique : RT-PCR (The BioTechniques Update Series)
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Binding: Paperback, 1 edition, 300 pages
Publisher: Eaton Publishing Company/Biotechniques Books
Weight: 1 pound
Dimension: H: 0.75 x L: 9.3 x W: 0.5 inches
ISBN 10: 1881299139
ISBN 13: 9781881299134
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Book Description:
This latest volume in the 'BioTechniques Update Series' continues the 'subseries' theme on PCR methodologies. Here are 39 key papers describing a broad spectrum of highly useful methods and applications for Reverse Transcription PCR technology. These papers first appeared in the journal BioTechniques from 1994 through Fall 1997, a period in which RT PCR rapidly evolved as a powerful, yet sensitive and discriminating analytical tool for the detection and study of gene expression, low abundance mRNAs and viral genomes. The papers are organized around three central themes: template preparation, gene expression analysis, and obtaining full length cDNAs and further grouped by functional topics such as isolating RNA from small tissue samples, removing contaminants, quantitative RT PCR, technology improvements and innovations. Most papers were reviewed and updated by their original authors to add information on further refinements and applications for the techniques described.

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